None, Conflict of Interest: Fourteen hours after the last dose of CCl4, rats were sacrificed, and the relative liver weight, weight gain, alkaline phosphatase, aspartate amino transferase, alanine aminotransferase, direct bilirubin, total bilirubin, triglycerides, total cholesterol, very low density lipoprotein, low density lipoprotein, high density lipoprotein, plasmatic glucose, urea, creatinine, acid uric and malondialdehyde were determined.
To observe the ameliorating effect by methanolic extract of pomegranate peel in acetaminophen-induced hepatotoxicity. The randomised controlled study was conducted from July to June at the University of Health Sciences, Lahore, Pakistan, and comprised rats that were randomly divided into three equal groups.
On day 15, blood was collected through cardiac puncture, and livers were removed and processed for histological examination. Each group had 8 Mean liver aspartate aminotransferase at the end of the experiment in groups A, B and C were Acetaminophen is hepatotoxic and methanolic extract of pomegranate peel ameliorated the hepatic picture probably because of its antioxidant properties.
Acetaminophen, Pomegranate peel, Hepatotoxicity. The animals were divided through random balloting into three equal groups A, B, and C and were individually housed in stainless steel cages with wood shavings on the floor.
The animals were fed on standard rat diet and water ad libitum. The photoperiod was controlled at 12 hours. The animals were then sacrificed under chloroform anaesthesia and the livers were removed, weighed and small pieces of mm were excised and prepared for histological examination.
Data were statistically analysed using SPSS The cell boundary of hepatocytes was not clearly defined. Cytoplasm contained large number of vacuoles pushing the nucleus to one side.
The nuclei were darkly stained, indicating pyknotic changes along with clumping of nuclear material. Nucleoli were not clearly seen in many hepatocytes.
Necrosis was present in group B as evident by pyknosis. The liver parenchyma showed vascular congestion Figure-2 In group C, general architecture of liver parenchyma was restored.
There was hardly any evidence of damage to the organ; no vascular congestion, necrosis or inflammation was observed. Signs of regeneration were present in group C as evident by cytoplasmic eosinophilia, prominent nucleoli, multinucleation and two cells thick liver cords Figure-3 similar to those seen in control group A.
There are several reports showing that acetaminophen administration increased the lipid peroxidation, disturbing cell membrane integrity and releasing the liver enzymes into circulation. This loss of classical arrangement of hepatocytes due to ballooning was also previously reported after acetaminophen treatment of albino rats.
Literature reports that increased cytoplasmic eosinophilia, multinucleated hepatocytes mostly binucleatedprominent nucleoli and two cell thick liver cords seen in group C animals are suggestive of regeneration.
However, further detailed studies are needed to establish and confirm these effects. However, treatment with MEPP reversed these changes, showing its ameliorating effects. MEPP deserves further intensive study as a protective and preventive agent since it has more potential being a rich source of antioxidants.
There had been no disclosure. There is no conflict of interest. University of Health Sciences, Lahore. Drug Metab Dispos ; What do we not know about how Paracetamol acetaminophen works? J Clin Pharm Ther ; Protection of acetaminophen induced hepatocellular apoptosis and necrosis by cholesteryl hemisuccinol pretreatment.
J Pharmacol Exp Ther ; Paracetamol in critical illness: Crit Care Resusc ; Effect of ribose cysteine pretreatment on hepatic and renal acetaminophen metabolite formation and glutathinone depletion.
Transcript of Effects of pomegranate and milk thistle extracts on chemically induced liver cancer in rats. Why Pomegranate? CONCLUSION To evaluate the anti-carcinogenic effect of pomegranate peel, milk thistle seed extracts. the present study suggested that PPE and MTSE are useful therapeutic agents in hepatocarcinogenesis. Study Type: Animal Study, In Vitro Study. Additional Links. Substances: Astragaloside. A methanolic extract of pomegranate peel ameliorated the hepatic damage caused by acetaminophen. Jun 30, Aged black garlic has hepatoprotective effects and suggests that supplementation might be a good adjuvant therapy for the management of liver. The present study was Trichloroacetic acid (TCA) is a prominent by-product of the chlorination of drinking water. It induces cell damage by producing free radicals and reactive oxygen species.
Basic Clin Pharmacol Toxicol ; The purpose of this study is to investigate the protective effect of Cynodon dactylon aqueous extract (mg/kg) in the liver tissue of streptozotocin induced diabetic rats. Thirty male albino wistar rats were divided into five groups.
The hepatoprotective effect of herbs was evaluated by measuring levels f o The histological studies were also carried Effect of soapworts, pomegranate peel and cloves, on liver enzymes of.
Biochemical Studies on The Hepatoprotective Effects of Pomegranate and Guava Ethanol Extracts Mohieldin Osman 1, The hepatoprotective effect of pomegranate and guava against carbon tetrachloride-induced Biochemical Studies On The Hepatoprotective Effects Of Pomegranate And Guava Ethanol Extracts.
Hepatoprotective effect was evaluated by comparing serum transaminase levels, bilirubin levels, albumin, and protein levels, coagulograms and pathohistological exams. We also measured flow rate by Doppler ultrasound of hepatic artery and portal vein before and after preconditioning. Transcript of Effects of pomegranate and milk thistle extracts on chemically induced liver cancer in rats.
Why Pomegranate? CONCLUSION To evaluate the anti-carcinogenic effect of pomegranate peel, milk thistle seed extracts. the present study suggested that PPE and MTSE are useful therapeutic agents in hepatocarcinogenesis. Protective Role of Lycopene Against Diethylnitrosamine Induced Experimental Hepatocarcinogenesis Inas Z.A.
Abdallah and Hala A.H. Khattab Nutrition and Food Science Dept., Faculty of Home Economics, Helwan University. Abstract Lycopene was considered as a major carotenoid in the human diet for only the last few centuries.